Sam Stosur defeated Venus Williams 6-3 4-6 6-3 on Friday, spoiling the chance of an all-Williams sister semi-final at the WTA event in Charleston, South Carolina.

Serena Williams reached the semis when Sabine Lisicki had to withdraw with a twisted ankle.

Venus hadn’t lost a set in three previous matches and had to get by the reigning US Open champion to set up her first meeting with her younger sister in nearly three years.

Venus Williams rallied from a set down and was up 2-1 in the third set before Stosur took control and won five of the final six games.

The win ended a long Friday for Stosur, who finished a rain-delayed third-round match before facing Venus.

The Australian will play Serena Williams on Saturday.