Roger Federer appeared to lose focus in his first match back after the birth of his second set of twins, allowing a lead to slip away in a second-round defeat at the Italian Open.

The 17-time Grand Slam winner lost to 47th-ranked Jeremy Chardy of France 1-6 6-3 7-6 (6).

Federer’s boys, Leo and Lenny, were born last week, prompting the fourth-ranked Swiss player to withdraw from the Madrid Open.

“This one is totally OK if it doesn’t hurt,” Federer said on Wednesday. “I tried everything. I can’t do more than that on the court.”

Meanwhile, Serena Williams showed no trouble – and no taping – from a left thigh injury that forced her to withdraw from Madrid.

The top-ranked American beat 28th-ranked Andrea Petkovic 6-2 6-2.

Wimbledon champion Andy Murray also eliminated Marcel Granollers of Spain 6-2 7-5.

Federer had a match point in the tiebreaker but Chardy saved it with an improbable cross-court forehand on the run when it looked like Federer was about to close the match out.

“That passing shot is a tough one for me to take, because he’s not going to make that very often,” Federer said.

“But it happens and credit to him to fight his way back into the match and get it.”

After cruising through the first set, Federer began to commit unforced errors at an alarming rate and Chardy quickly took advantage amid swirling wind at the Foro Italico.

“I wasn’t able to stretch the lead when I had it,” Federer said.

Federer committed 43 unforced errors – 20 in the second set alone – to Chardy’s 28.

Chardy hadn’t beaten a top-10 player since defeating Juan Martin del Potro in the third round of last year’s Australian Open.

Along with his recently-born twin boys, Federer and wife Mirka also have twin girls who turn 5 in July.

But even with all the family distractions, Federer didn’t appear worried about his preparations for the French Open, which starts in 11 days.

“Everything is under control,” Federer said, recalling that he reached the final of the Monte Carlos Masters last month.

“I still feel good, my body is good, my mind is good and it’s just unfortunate for one passing shot today I don’t get another opportunity to play this week.”

Also advancing was 15th-seeded Tommy Haas, who eliminated Igor Sijsling of the Netherlands, 7-6 (6) 6-1.

In women’s play, Australian veteran Sam Stosur defeated Elena Vesnina of Russia 6-2 6-3.