A teenager on first-class debut denied Michael Clarke much-needed time in the middle at Derby on Thursday.
Centurions David Warner and Shaun Marsh started the three-day clash with Derbyshire by sharing a 154-run stand.
Australia reached 9-413 at stumps on day one after Clarke won the toss.
The skipper, who has scored two centuries in his past 26 Test innings, struggled to get going in the Ashes tour game.
He was out for 16 off 37 balls during the second session, paceman Will Davis finding the edge and Tom Knight snaffling a catch at slips.
The 19-year-old Davis also dismissed Adam Voges and Peter Nevill, both for one.
Warner picked up where he left off at Lord’s, celebrating his first ton on the Ashes tour before retiring on 101 off 108 balls.
Marsh was more methodical in the town that helped launch the industrial revolution, taking over four hours and 177 deliveries to reach three figures and also retire on 101.
The Western Australian all but confirmed his status as the frontrunner to replace Chris Rogers in the third Test at Edgbaston, should the opener fail to prove his fitness before Wednesday.
Rogers is undergoing further tests on his vestibular system, having retired hurt on day four of the second Test due to an alarming dizzy spell.
Nevill batted at No.6, two spots above Brad Haddin, suggesting the 29-year-old is well placed to keep his spot in the Test XI.
Nevill impressed in Australia’s 405-run win at Lord’s, scoring a polished 45 and snaring seven catches to equal Brian Taber’s Australian record for most dismissals on Test debut.
Haddin, who withdrew from the second Test due to family reasons, is set to share wicketkeeping duties with Nevill in the clash with Derbyshire.
Haddin received treatment from physio Alex Kountouris for what looked to be cramp, but recovered to score 32 before his stumps were rattled by Tony Palladino.
Mitch Marsh and Shane Watson were both caught behind down the leg side, having scored 53 and 61 respectively.
Harry White, aged 20 and on first-class debut, dismissed both allrounders.
Watson, who was replaced by understudy Marsh for the second Test, teed off and needed only 39 balls to bring up his half-century off 39 balls.
Pacemen Mitchell Johnson, Mitchell Starc and Josh Hazlewood, offspinner Nathan Lyon and in-form batsman Steve Smith were rested from the fixture.