Mike Brown was the most high-profile absentee from a 25-man squad named on Tuesday by England coach Eddie Jones for their Twickenham clash against South Africa.

Brown, 33, has been a regular under former Wallabies boss Jones, usually at fullback but playing on the wing with some success in the June tour of South Africa.

However, he was not among the group of 25 who will remain in the team’s training camp in Portugal.

His absence means Elliot Daly is likely to start at fullback, with Jonny May, Jack Nowell and Chris Ashton competing for the wing berths for this weekend’s showdwon in London.

Also missing is Ben Morgan, who was recalled to the squad to cover the absence of Billy Vunipola.

That means a likely start at number eight for Zach Mercer, one of three uncapped players in the group, which will be trimmed to 23 on Thursday morning.

Lock Courtney Lawes has been ruled out with a back injury but will stay in Portugal.

Following the Springboks Test, England then face New Zealand, Japan and then Australia on November 24.

England 25-man squad:

Forwards: Tom Curry, Charlie Ewels, Jamie George, Dylan Hartley (co-capt) Alec Hepburn, Maro Itoje, George Kruis, Zach Mercer, Ben Moon, Brad Shields, Kyle Sinckler Elliott Stooke, Harry Williams, Mark Wilson.

Backs: Chris Ashton, Danny Care, Elliot Daly, Owen Farrell (co-capt), George Ford, Jonny May, Jack Nowell, Henry Slade, Ben Te’o, Manu Tuilagi, Ben Youngs.